a novel method of purification of specific hydatid cyst antigen

نویسندگان

z zamani

m assmar from the departments of parasitology and biochemistry, pasteur institute of iran, tehran, i.r. iran.

n pyazak

k wafaie

چکیده

hydatid cyst fluid has been used as a source of antigen for the serodiagnosis of echinococcus granulosus infection. due to cross-reactions with antigens shared by other helminthes, the specific antigen from hydatid cyst fluid was purified by many workers. we used a relatively simple technique for purification of specific antigen from sheep hydatid cyst fluid. isopycnic ultracentrifugation with 30% ker was used followed by sds-page to check the purity of the antigen. the antigen was of 48 kda and used in elisa and ira with high sensitivity and specificity.

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A NOVEL METHOD OF PURIFICATION OF SPECIFIC HYDATID CYST ANTIGEN

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عنوان ژورنال:
medical journal of islamic republic of iran

جلد ۱۵، شماره ۱، صفحات ۴۹-۵۳

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